PEGFP N2 PDF

3 Oct Description: pEGFP-N2 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher. Provide pEGFP-N2 vector/plasmid map, full length sequence, antibiotic resistance, size and other information. pEGFP-N2 encodes a red-shifted variant of wild-type GFP which has been optimized for brighter fluorescence and higher expression in mammalian cells.

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Sticky ends from different SfiI sites may not be compatible. The vector backbone also contains an SV40 origin for replication in mammalian cells expressing the SV40 T-antigen.

Partner with Takara Bio! Sticky ends from different PflFI sites may not be compatible. Measure NFkB activity with a ZsGreen1 reporter that has a high signal-to-noise ratio and a bright signal.

Efficient cleavage requires at least two copies of the XmaI recognition sequence. Our brands Takara Clontech Cellartis.

The coding sequence of the EGFP gene contains more than silent base changes which correspond to human codon-usage preferences 5. The coding pegfp n2 of the EGFP gene pegfp n2 more than silent base changes which correspond to human codon-usage preferences 5.

Sequences flanking EGFP have been converted to a Kozak consensus translation initiation site 6 to further increase the pegfp n2 efficiency in eukaryotic cells. Sticky ends from different TthI sites may not be compatible. This plasmid has been discontinued by Clontech. Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy. An b2 with takarabio.

Looking for EGFP vectors? Upgrade to AcGFP1 or ZsGreen1 vectors

Label the inner leaflet of the plasma membrane with AcGFP1 and select with either G or hygromycin. Vectors for expressing and visualizing a protein pegfp n2 interest fused to AcGFP1, including a pre-linearized vector for simple, one-step In-Fusion cloning. Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. Pegfp n2 brands Takara Clontech Pegfp n2. Deliver and stably express your gene of interest fused to AcGFP1, in a broad range of dividing cells.

This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin.

pEGFP-N2 vector

If required, stable transformants can be selected using G pegfp n2. Sticky ends from different BsaI sites may not be compatible. EGFP has been reported to form n Jain pehfp al. Efficient cleavage requires at least two copies of the SfiI recognition sequence. Efficient cleavage requires at least two copies pegfp n2 the XmaI pegfp n2 sequence. Oligomerization of green fluorescent protein in the secretory pathway of endocrine cells.

Get Snap Gene Viewer. Welcome to the new website for Clontech, Takara and Cellartis products – any questions please contact webmaster takarabio. There combinant EGFP vector can be transfected into mammalian cells using any standard transfection method. EGFP has been reported to form dimers Jain et al.

Oligomerization of green fluorescent protein in the secretory pathway of endocrine cells. Create an account to get started.

PEGFP N2 DOWNLOAD

Get Snap Gene Viewer. Express a rapidly degraded form of ZsGreen1 in studies that require rapid reporter turnover. All pegfp n2 are the property of Takara Bio Inc. Sequences flanking EGFP have been converted to a Kozak consensus translation initiation site 6 to further increase the translation efficiency in eukaryotic cells.

ZsGreen1 has been reported to have better expression than EGFP in lung and muscle tissue and in cultured cells Bell et al. Use with any Living Colors vector that contains a neomycin resistance cassette to create double-stable cell lines. Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy.

Looking legfp EGFP vectors? Measure NFkB activity with a ZsGreen1 reporter that has a high signal-to-noise ratio and a bright signal. Fusions pgefp the N terminus of EGFP retain the fluorescent properties of the native protein allowing the localization of the fusion protein in vivo.

PEGFP N2 DOWNLOAD

Additional product, intellectual property, and restricted use information is available at takarabio. Sticky ends from different RsrII sites may not be compatible.

The 1-base overhangs produced by TthI may be hard to ligate. Nn2 cleavage requires at pegfo two copies of the SfiI recognition sequence. Our mission is to develop high-quality innovative tools and services to accelerate discovery. Mapping the brain, one cell type at a time Learn about pioneering efforts to map the mammalian brain using single-cell transcriptomics.